Droplet Digital PCR
The D30 Automatic Digital PCR (ddPCR) Platform represents the next generation of nucleic acid quantification technology, engineered to deliver absolute precision in molecular analysis. Unlike traditional PCR or qPCR, which rely on standard curves and relative quantification, the D30 uses droplet-based digital PCR target nucleic acids are randomly distributed into thousands of uniform water-in-oil (WO) droplets, amplified to endpoint via PCR, and quantified through direct molecular counting. This approach eliminates the need for calibration standards, ensuring unparalleled accuracy—even for low-abundance targets. Developed for both research and clinical labs, the D30 sets a new benchmark for sensitivity, reproducibility, and efficiency in nucleic acid analysis.
Specifications
Reaction Volume
Up to 50μL (larger volume enhances sensitivity for low-abundance targets)
Microdroplet Count
Up to 100,000 per reaction (ensuring robust statistical power)
Quantification Limit
500,000 copies per system
Temperature Control
Innovative flat-tube 3D design with ≥10°C/s heating/cooling rates
Workflow
Fully enclosed chip (droplet generation → PCR amplification → detection)
Certification
CE
Consumables
Digital PCR Universal Kit (960 tests/kit); Microfluidic chips (8 tests/pc, 12 pcs/box)
Key Features
Unmatched Sensitivity & Accuracy
Fully Enclosed, Contamination-Free Workflow
With a reaction volume of 50μL—larger than many conventional ddPCR systems—and up to 100,000 microdroplets, the D30 excels at detecting rare targets, such as low-frequency mutations or minimal residual disease. Its high partitioning count ensures precise quantification even for samples with extreme target variability.
The D30 utilizes a proprietary microstructure multiplexing technology, where all steps—droplet generation, PCR amplification, and detection—occur within a single enclosed chip. This eliminates environmental exposure, drastically reducing the risk of cross-contamination—a critical advantage for high-throughput labs and clinical settings.
Rapid Turnaround with Advanced Thermocycling
Versatile & Scalable
Equipped with a unique flat-tube 3D temperature control system, the D30 achieves heating and cooling rates exceeding 10°C/s. This cuts down PCR run times, accelerating workflow without compromising amplification efficiency—ideal for labs balancing speed and accuracy.
Compatible with a range of applications, the D30 supports both research and clinical use cases. Its consumables (universal kits and microfluidic chips) are optimized for consistency, ensuring reliable results across runs and operators.
Advantages
Absolute Quantification: No standard curves required—direct counting delivers definitive results.
Superior Sensitivity: Detects rare mutations and low-abundance targets that qPCR might miss.
Minimal Contamination Risk: Enclosed chip design protects against carryover, a common pitfall in molecular labs.
Fast & Efficient: Rapid thermocycling reduces time-to-result, increasing lab throughput.
CE-Certified: Meets rigorous international standards for clinical and research use.
Applications
The D30 is a versatile tool across diverse fields:
Genetic Analysis: Copy number variation (CNV) detection, allele imbalance, and single-cell gene expression profiling.
Rare Mutation Detection: Identifying low-frequency somatic mutations in cancer (e.g., liquid biopsy for minimal residual disease).
Validation & Confirmation: Verifying next-generation sequencing (NGS) results with high precision.
Clinical Diagnostics: Genetic disease screening, pathogenic microbial detection (e.g., viral load quantification), and non-invasive prenatal testing.
Environmental & Agricultural Testing: GMO detection, environmental monitoring, and species identification in food/agricultural samples.
The D30 Digital PCR Platform is a combination of sensitivity, speed, and contamination control makes it indispensable for labs demanding the highest standards of accuracy.